FastQCFastQC Report
Mon 27 Feb 2023
SRR3126503.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126503.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414742
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123130.8703353685689688No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35680.252201461467886No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25230.17833640338662454No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG23820.16836992186561223No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG18970.13408805280397415No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACAGCGAA18080.1277971531204983No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC16540.11691177614010187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT38850.085.752631
ATAGCGG3200.085.287071
TAGGGTA5300.080.6925054
TAGGGCA7700.077.514094
GACCGAT800.076.369698
AGGGATG26950.075.857435
GTACGGG4400.074.7992552
AGTAGGG22650.074.5209052
GAATAGG7900.074.454011
CGTAGGG3500.073.883342
GGTAAGG6500.073.840061
ATAGGGC8600.072.1345143
AGTACGG2500.071.5235061
AGGGTAC4950.071.207175
GAGGGAT17450.070.831754
TAGTCGG409.673204E-870.5824051
GTAGGGT5150.070.266963
AGGGACT24800.069.926585
AGGGAAT17650.069.762855
AGATAGG8350.069.314461
Produced by FastQC (version 0.11.5)