FastQCFastQC Report
Mon 27 Feb 2023
SRR3126502.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126502.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458140
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124040.8506727749050159No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34590.23722002002551196No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG27400.18791062586582907No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25540.17515464907347716No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG20820.1427846434498745No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC18640.12783409000507495No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACAGCGAA17490.11994733016034126No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT40950.083.9997251
AGGGATG24600.078.321675
ATAGCGG3200.077.936391
GGTAAGG7400.077.5787051
TAGGGCA6650.077.026124
ATAGGGC8550.075.8483663
GAATAGG8000.074.7012941
TAGGGAT9900.073.100234
AGTAGGG24500.072.69532
CGTAGGG4100.072.208762
AGGGAAT17750.070.688075
AGGGCAT12300.070.298485
AAGGGTA9150.069.847534
ACGGGAT4850.069.762814
AGAGGGC21350.068.893733
TAGGGTA5000.068.609794
GTACGGG4400.068.353462
TAAGGGA13250.067.740863
GAGGGAT16700.067.534864
AGGGACT27150.067.503775
Produced by FastQC (version 0.11.5)