FastQCFastQC Report
Mon 27 Feb 2023
SRR3126501.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126501.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632344
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161370.9885783878888273No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40840.2501923614140157No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30350.18592894634954396No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG29530.18090549541028117No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG25750.15774861181221606No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC21300.13048720122719232No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT51900.084.482551
TAGGGCA8100.077.749744
AGGGATG28700.077.45665
TAGGGTA6550.076.057794
CGTAGGG4700.075.996732
TAGGGAT11400.074.207344
ATAGCGG3900.072.377751
AGGGCAT16450.071.71125
GAGGGAT18950.071.427014
AGTAGGG27700.071.0908052
GGTAAGG8000.070.56831
AGGGACT25350.070.265225
GTACGGG4550.069.205812
ATAGGGC10500.068.930373
GAATAGG8750.068.820891
GTAGGGA13400.068.743313
AGGGATA13450.068.487765
ATGCGGG9300.067.7175142
AGGGTAC5500.067.506185
ATAGGGA14100.067.330443
Produced by FastQC (version 0.11.5)