FastQCFastQC Report
Mon 27 Feb 2023
SRR3126500.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126500.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213873
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTACATCA30231.41345564891314No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG16310.7626021049875393No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC15670.7326778041173968No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14720.6882589200132789No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATG12810.5989535846039472No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA10910.5101158163957115No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG8830.41286183856774816No Hit
GAATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT8430.39415915052390904No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCCTACATCATC7510.35114296802307915No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCCTACATCATCG7460.3488051320175992No Hit
CGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT7120.332907847180336No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6450.3015808447069055No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG5770.269786275032379No Hit
GATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC5520.25809709500497957No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG5480.25622682620059567No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGC5260.24594034777648419No Hit
AAATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT4960.23191333174360484No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCCTACATCATCGT4760.22256198772168528No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCC4590.21461334530305368No Hit
ACTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG4520.21134037489538185No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG4510.21087280769428585No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC3980.18609174603619907No Hit
GACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC3920.1832863428296232No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT3820.17861067081866341No Hit
ATCCGTACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCG3570.16692149079126398No Hit
GACACACCCACACCTGTCTCTTATACACATCTGACGCCTACATCATCGTA3420.15990798277482432No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATG3240.1514917731550967No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG3050.14260799633427312No Hit
AATGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT2960.13839989152440935No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG2930.13699718992112142No Hit
AGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT2890.1351269211167375No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT2680.12530800989372198No Hit
GACACACCCACACACCGAGATCCTGTCTCTTATACACATCTGACGCCTAC2510.11735936747509035No Hit
ATCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC2380.11128099386084264No Hit
GACTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT2320.10847559065426679No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGG2310.1080080234531708No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG2260.10567018744769092No Hit
ACACCGAGATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGC2250.10520262024659494No Hit
ACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT2240.10473505304549896No Hit
GACACACCCACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATG2230.10426748584440299No Hit
GGCAAGGTAATTGCTACATAGATTCTTTTCTTATTATTGCTGATTCTTAT2150.10052694823563517No Hit
AAATGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT2140.10005938103453918No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGG156.863021E-494.108151
TTGTAGG156.863021E-494.108151
TCATAGG156.863021E-494.108151
ATTGCGG201.8240156E-594.108151
AGTACGG201.8240156E-594.108151
GACTGGG254.8875154E-794.064112
AATCGGG301.3144017E-894.064112
GTACGGG301.3144017E-894.064112
GTACCCC254.907997E-793.998135
CGGGTAG201.8346438E-593.998125
GGTAACT156.8950135E-493.998128
AGGGTAC409.094947E-1293.998125
ACGGGAT156.8950135E-493.998124
CAGGGTA409.094947E-1293.998124
ATCGGGG301.3209501E-893.998123
CGTTTTT12950.090.474631
TAGTTGG500.084.6973341
TAGTAGG500.084.6973341
GCATGGG452.7284841E-1183.612532
ATAGGGC1300.083.152193
Produced by FastQC (version 0.11.5)