FastQCFastQC Report
Mon 27 Feb 2023
SRR3126499.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126499.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218360
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTACATCA27611.2644257189961532No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG17510.8018867924528302No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC15430.7066312511448983No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14930.6837332844843378No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATG10570.4840630152042499No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG10180.46620260120901263No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA10170.46574464187580145No Hit
GAATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT9300.4259021798864261No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCCTACATCATCG7800.35720827990474446No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCCTACATCATC7200.3297307199120718No Hit
CGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT6710.30729071258472246No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6370.2917200952555413No Hit
GATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC6120.280271111925261No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG5730.2624106979300238No Hit
AAATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT5670.25966294193075656No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCC5480.25096171459974354No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG5360.24546620260120902No Hit
ACTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG5130.23493313793735118No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGC5120.23447517860413994No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT4870.22302619527385967No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG4710.21569884594248032No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCCTACATCATCGT4590.2102033339439458No Hit
GACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC4540.20791353727788975No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC3950.18089393661842829No Hit
GACACACCCACACCTGTCTCTTATACACATCTGACGCCTACATCATCGTA3570.16349148195640226No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATG3340.15295841729254442No Hit
AATGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT3260.14929474262685472No Hit
AGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT3080.14105147462905296No Hit
ATCCGTACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCG3080.14105147462905296No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG2970.13601392196372963No Hit
ATCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC2760.1263967759662942No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG2710.12410697930023815No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT2670.1222751419673933No Hit
ACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT2660.12181718263418208No Hit
GACTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT2600.11906942663491482No Hit
GACACACCCACACACCGAGATCCTGTCTCTTATACACATCTGACGCCTAC2420.11082615863711302No Hit
AAATGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT2320.10624656530500091No Hit
ACACCGAGATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGC2290.10487268730536728No Hit
GGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT2280.10441472797215606No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGG2250.10304084997252243No Hit
GGAACTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT2240.10258289063931124No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAGG301.3073077E-894.139691
ACGTAGG156.854079E-494.139691
TCATAGG156.854079E-494.139691
ATCGCGG301.3073077E-894.139691
GCGTAGG156.854079E-494.139691
TGGTCGG156.854079E-494.139691
TCGATGG201.8210685E-594.139681
TAGTAGG450.094.139681
CGTAGGG353.583409E-1093.9887852
GGTAAAC201.8356368E-593.988786
AGTGGTA156.897935E-493.988789
GGTATAA156.897935E-493.988787
TTCGGCA156.897935E-493.988788
CGTCCAC156.897935E-493.988784
AGGGACC650.093.988785
CGTTTTT13050.090.893481
AGGGAAT6500.088.204865
AGGGATG1200.086.156385
ATAGAGG1400.084.053281
TAAGAGG900.083.679721
Produced by FastQC (version 0.11.5)