FastQCFastQC Report
Mon 27 Feb 2023
SRR3126498.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126498.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234329
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG20280.8654498589589851No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19520.8330168267692006No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC17350.740411984858895No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG11710.4997247459768104No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTACATCA10610.4527821993863329No Hit
GAATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT10080.43016442693819373No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCCTACATCATCG8040.34310734053403547No Hit
CGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT7680.32774432528624287No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6900.2944577922493588No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCC6580.28080177869576534No Hit
AAATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT6490.2769610248838172No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG6430.2744005223425184No Hit
GATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC6090.2598910079418254No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG5630.24026048845853482No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG5250.2240439723636426No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT4970.2120949605042483No Hit
GACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC4740.20227970076260302No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA4570.19502494356225647No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC4410.18819693678545976No Hit
AATGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT4200.17923517789091406No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATG3930.16771291645506958No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATG3820.16301866179602184No Hit
GACACACCCACACCTGTCTCTTATACACATCTGACGCCTACATCATCGTA3810.16259191137247203No Hit
ACTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG3360.14338814231273123No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG3360.14338814231273123No Hit
AGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT3280.13997413892433289No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT3220.1374136363830341No Hit
GACACACCCACACACCGAGATCCTGTCTCTTATACACATCTGACGCCTAC3080.13143913045333697No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCCTACATCATC2840.12119712028814188No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGG2760.11778311689974352No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG2750.11735636647619371No Hit
GACTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT2710.11564936478199454No Hit
AAATGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT2700.11522261435844475No Hit
GGCAAGGTAATTGCTACATAGATTCTTTTCTTATTATTGCTGATTCTTAT2660.11351561266424556No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCCTACATCATCGT2640.11266211181714597No Hit
ACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT2630.11223536139359618No Hit
GGAACTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT2610.11138186054649658No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG2580.11010160927584721No Hit
ATCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC2560.1092481084287476No Hit
ACACCGAGATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGC2480.10583410504034925No Hit
GACACACCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCG2450.10455385376969986No Hit
GGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT2430.10370035292260027No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT2370.10113985038130151No Hit
GACACTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT2360.10071309995775171No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAGG500.094.116241
CTAACGG156.8615016E-494.116241
GTGTAGG301.3100362E-894.116241
TATAGAG156.8615016E-494.116241
CAGGGTA201.8352446E-593.995734
GTAAAGG950.089.162751
CGTAGGG850.088.466572
CGTTTTT17800.088.03571
GTAGGGT600.086.162763
CTAGAGG550.085.560221
GGATAGC500.084.596167
TAGGGCA950.084.101444
CGGGAAT950.084.101445
ACGTAGG452.7284841E-1183.658881
AGTTAGG401.020453E-982.351711
TGCAAGG800.082.351711
GGACTTT401.031367E-982.246267
AGGGTAT401.031367E-982.246265
GATGGGC401.031367E-982.246263
GTAGGGC750.081.4629753
Produced by FastQC (version 0.11.5)