FastQCFastQC Report
Mon 27 Feb 2023
SRR3126497.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126497.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2475103
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262631.061087154756792No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40180.16233667851398506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT91450.086.362541
CGTAGGG7550.072.2477342
AGGGATG34100.071.6665345
AGTAGGG44650.070.561292
ATAGCGG6450.070.053311
ATAGGGC12750.070.03433
ATAGGGA19700.069.182973
ATAAGGG33550.069.098332
TAGCGGG14450.068.9892042
TAGGGCA11050.068.9001544
CGAAGGG23700.068.6500552
TAGACGG3500.068.583441
ATAGGGT11150.067.860723
AATAGGG35500.067.819532
GTAGGGA21700.067.787873
GGTAAGG14550.067.2848741
TAAGGGA23550.066.453943
ACGGGTA2900.066.443624
AGAGGGC36500.066.439183
AGGGACT33600.066.159065
Produced by FastQC (version 0.11.5)