FastQCFastQC Report
Mon 27 Feb 2023
SRR3126496.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126496.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518324
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264391.0498649101545312No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37940.15065575358849775No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94350.086.4343261
ATAGGGC13500.076.929433
ATAGGGT12100.073.79083
AGTAGGG41750.072.598662
CGTAGGG8600.072.1274952
TAGGGTC7350.069.690434
ACGGGTA2850.069.2529754
ATAAGGG35050.068.7788242
TAGCGGG13900.068.628842
AGGGTAC13100.068.516645
GATAGGG37700.068.30692
GTAGGGT10000.068.1399843
TAGGGTA9450.067.630274
GGTAAGG14350.067.186351
ATAACGG2250.066.887741
ATAGAGG16200.066.481311
TAAGGGA22850.066.427873
GAGGGAT29700.066.138434
AATAGGG36050.065.697772
ATAGGGA20850.065.587483
Produced by FastQC (version 0.11.5)