FastQCFastQC Report
Mon 27 Feb 2023
SRR3126495.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126495.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814059
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330741.1753129554142256No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44040.15649991702377242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT108050.086.0181351
CGTAGGG8600.072.683132
ATAGGGT13200.072.633453
ATAGGGC15400.072.633453
GTAGGGC14250.072.5584953
AGTAGGG49600.072.1079942
TAGGGTA11800.071.692054
ATAACGG2650.070.997321
TAGGGCA14550.069.124384
ATAGCGG7150.069.073441
AGGGATG40150.068.477965
ATAGGGA24650.068.447563
ACGGGAT7000.067.811574
CGAAGGG28100.067.5702442
TAGCGGG16350.067.550812
ATGCGGG18100.067.511112
TAGGGAT18800.067.49734
ACGGGTA3150.067.140174
TAGAGGG50100.066.41652
GTAGGGA23650.066.174943
Produced by FastQC (version 0.11.5)