FastQCFastQC Report
Mon 27 Feb 2023
SRR3126494.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126494.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926362
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235111.2204871150905179No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27070.1405239513653197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84500.085.939341
TAGGGCA12350.071.160184
ATAGGGC12300.070.685283
AGGGATG34050.070.666775
TAGGGCG4100.068.7748644
GTAGGGC10700.068.517763
AGTAGGG33600.067.877242
AGAGGGC32750.067.588373
ATAGAGG16900.067.392481
TAGAGGG41550.067.339162
AGGGAAT22000.065.5815
AAGGGAT34550.065.155314
ATAGCGG4050.065.075241
AAGAGGG71650.064.711642
GATAGGG25850.064.5787352
AAGGGGC56500.064.5469364
GAGGGAT24400.064.5234154
ATAAGGG29100.063.991742
ATGCGGG10850.063.710252
ACTAGGG10950.063.557862
Produced by FastQC (version 0.11.5)