FastQCFastQC Report
Mon 27 Feb 2023
SRR3126493.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126493.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1958019
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234331.196770817852125No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26830.13702624948991812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81900.086.26561
ATAGGGC12150.070.784493
CGTAGGG4950.070.253452
AGGGATG31200.069.5906145
ACGGGAT5800.068.8738254
AGTAGGG33800.068.127192
TAGGGCA11250.068.0923544
AGAGGGC33450.068.0005653
ATGCGGG10450.067.9052
TAGAGGG39150.067.822
AAGAGGG70550.067.210212
TAGGGCG4150.066.813964
GAGGGAT23650.066.371044
ATAGAGG16400.065.1078951
AAGGGGC57450.064.624974
GTAAAGG16050.064.476181
TAGGGAT13650.064.038864
GTAGGGC10950.063.9492453
AAGGGCA31100.063.7698444
ATAAGGG29850.063.760512
Produced by FastQC (version 0.11.5)