FastQCFastQC Report
Mon 27 Feb 2023
SRR3126491.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126491.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3189913
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274210.859615920559589No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG88880.27862828860849814No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG69760.2186893498349328No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42010.13169638168815262No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTATAGGAC40750.12774643070202857No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31930.10009677379916003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90550.083.155051
CGTAGGG6750.072.43992
AGGGCAT29900.071.986655
GGTAAGG18650.070.401591
TAGGGCA21150.070.215734
TAGCGGG17300.069.301462
GGGCGAT8550.069.2567446
AGGGAAT29900.068.685955
GTACGGG9250.068.618362
AGTAGGG49400.068.335412
GATAGGG43100.068.0699842
AAGGGTA25450.067.4004444
AGGGATG47750.067.024165
ATAGGGA25400.066.978063
ATAGGGC22250.066.7443853
TAGGGAC15450.066.61524
GTAGGGA26200.066.3679053
AGTACGG5750.066.2940141
GAGGGAT43850.065.91184
CGAAGGG31750.065.896842
Produced by FastQC (version 0.11.5)