FastQCFastQC Report
Mon 27 Feb 2023
SRR3126490.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126490.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3275176
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283050.8642283651321334No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG93740.2862136263822158No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG78190.23873526186073665No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41940.12805418701162932No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTATAGGAC36480.111383327186081No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT91250.083.501761
CGTAGGG6650.079.851962
AGGGATG46550.072.584625
AGGGCAT32150.071.330335
TAGGGTA10300.070.261744
ATAGGGA26750.069.5675663
GGTAAGG18100.069.38191
TAGGGCA18650.069.0410164
TAGCGGG18400.068.7010352
AGTAGGG52350.068.581262
AAGGGTA24450.068.4237064
AGGGAAT35700.068.186285
GAGGGAT40950.067.477484
GGATAGG17500.066.9229051
AGGGTAT17950.066.759215
ACGGGTA3100.066.700094
GACCGAT3050.066.2558068
AGACGGG18350.066.0712362
GTAGGGA25900.065.863123
ATAGGGC21700.065.6172943
Produced by FastQC (version 0.11.5)