FastQCFastQC Report
Mon 27 Feb 2023
SRR3126489.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126489.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3647532
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365441.0018829169970271No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG107370.29436342162316875No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG81600.22371291053786507No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT49580.13592752579004105No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36940.10127395729495998No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT112650.083.4591141
CGTAGGG8650.075.524372
AGGGCAT35150.073.675085
ACGGGTA5100.072.803344
TAGGGCA23950.072.412784
AGTAGGG60050.071.7702942
GGTAAGG23500.070.447721
TAGGGTC10350.069.02364
AGGGAAT36900.068.779945
TAAGGGA32050.068.775413
TAGAGGG54300.068.7241062
ATAGGGA30250.068.517523
ATAGGGC24350.068.327063
AGGGATG48600.068.178475
GTAGGGC16300.067.4709243
AAGGGTA27350.067.1914144
TAGCGGG18750.066.9265442
TAGACGG4100.066.5328751
AGACGGG19500.066.521632
AGAGGGC52500.066.514753
Produced by FastQC (version 0.11.5)