FastQCFastQC Report
Mon 27 Feb 2023
SRR3126488.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126488.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2499095
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240400.9619482252575433No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT48150.19266974644821427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80550.084.384421
ATAGGGC15150.074.760013
TAGGGCA11600.074.5460744
GAGGGAT25850.074.3584
AGTAGGG42450.072.662182
GGTAAGG13150.072.307981
AGGGATG31900.071.452265
TAGGGTC6850.069.9800954
TAAGGGA20800.069.138993
GAATAGG14650.068.438841
ATAGCGG6600.067.754841
ATAGGGA18300.067.541333
CGTAGGG7800.067.515842
AGATAGG12150.067.411441
AGTAAGG16600.067.2048951
GTAGGGA19250.067.137773
ATAAGGG36850.066.989082
AGAGGGC39700.066.7657243
ATGAGGG38450.065.913542
AGGGAAT21000.065.347435
Produced by FastQC (version 0.11.5)