FastQCFastQC Report
Mon 27 Feb 2023
SRR3126487.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126487.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2556989
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243620.9527612359693374No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT48560.18991086782148847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79850.084.454231
GTACGGG5250.076.980472
TAAGGGA21850.071.6213763
ATAGGGC15250.071.4937443
AGTAGGG43100.071.417632
GGTAAGG15450.071.225361
AGGGAAT23800.071.084595
GAATAGG14800.070.222751
TAGGGCA12500.069.9282764
GAGGGAT23200.069.884524
CGTAGGG7550.068.467922
ACGGGAT7650.068.1885454
GTAGGGA19250.067.867833
ATAAGGG38300.067.85282
AGGGTAC10750.067.759965
AGGGATG30650.067.157355
ATAGGGA21050.066.975973
TAGGGTC6550.066.725464
AAGGGGC79300.066.669814
AGGGACT40850.065.804245
Produced by FastQC (version 0.11.5)