FastQCFastQC Report
Mon 27 Feb 2023
SRR3126486.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126486.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863656
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309971.0824274982749325No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53900.18822093156440578No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98000.085.037841
CGTAGGG8000.073.436832
AGTAGGG46650.073.043282
TAGGGCA14850.072.792684
GTAGGGA21400.071.596233
ATAGGGC18400.071.008983
ATAGGGA23950.069.467883
ATAGAGG19300.069.448381
GGTAAGG14000.069.2012251
ATAAGGG43450.068.0385062
AGGGCAT24400.067.8014765
ATAGCGG7100.067.564161
ACGGGTC3550.067.519334
GAATAGG18950.067.504741
GTAGGGT10400.066.882843
GAGGGAT27000.066.842674
GTACGGG6050.066.809312
GTAGGGC13300.066.7877043
AGGGATG36000.066.712115
AGTAAGG16500.066.697031
Produced by FastQC (version 0.11.5)