FastQCFastQC Report
Mon 27 Feb 2023
SRR3126485.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126485.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683220
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146380.869642708618006No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC42110.2501752593243901No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG38280.22742125212390535No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG33590.19955799004289396No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC32630.19385463575765496TruSeq Adapter, Index 13 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATGTGCTT26460.1571987024869001No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25800.1532776464157983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT5800.085.080964
CGTTTTT49200.084.280561
GTACGGG3850.076.959222
CGTAGGG4800.076.4247742
AGGGATG30650.075.134035
GTAGGGA14200.074.136283
ATAGCGG2800.073.962451
AGGGAAT22750.073.5427255
TACGACC9950.072.7392356
AGTAGGG25850.072.5927352
TAGGGCG2400.072.4538654
AAGGGCG6250.072.187554
TAGGGTA8100.071.946184
GTAGGGT8050.071.809243
GGTAAGG8300.070.8840561
GAGGGAT24750.070.827964
AGGGCAT14550.070.7379155
CTACGAC10300.070.267525
CGCTACG10250.070.151773
TAGCGGG8650.069.594462
Produced by FastQC (version 0.11.5)