FastQCFastQC Report
Mon 27 Feb 2023
SRR3126483.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126483.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920301
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187520.9765135778193107No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC49920.2599592459723762No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG45520.2370461714075033No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG40180.2092380309128621No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC39870.20762370065942787TruSeq Adapter, Index 13 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28880.15039308941671126No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT20620.10737899943810893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT63650.084.4678961
ACGGGAT6200.076.565684
AGGGAAT24550.075.239435
TAGGGCG3750.075.201144
AGGGATG34450.074.628135
GTACGGG4350.074.5528642
ACGGGTC1400.073.858274
AGTAGGG31100.072.087912
AAGGGCG8550.072.0127944
GTAGGGA15550.071.634533
CGTAGGG5450.071.579072
AGGGCAT16350.071.29165
AAGGGAC17050.070.845664
TAGGGCA10950.070.8234
TAAGGGA16050.069.98863
ACTAGGG8800.069.966972
AGGGTAC10350.069.933435
GAGGGAT27000.069.804774
GGAATGC14100.069.66417
GAGGGTA12200.069.3453144
Produced by FastQC (version 0.11.5)