FastQCFastQC Report
Mon 27 Feb 2023
SRR3126482.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126482.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3284597
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311440.9481832931102354No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG38810.11815757001543872No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35220.10722776645049606No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG33410.10171719696510713No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96050.085.268341
GTACGGG7700.076.944752
TAGGGCA18850.072.299364
AGTAGGG59200.071.803542
ACGGGAT9350.069.863624
ATAGGGC21000.069.8205263
TAGGGTA11250.069.760864
GTAGGGA27900.069.73393
ATAGCGG6150.069.646971
GGTAAGG20450.069.510371
CGTAGGG8900.068.683512
GTAGGGC15650.068.164673
AGGGATG49450.067.37955
TAGACGG5500.066.752551
AGGGCAT33450.066.312255
AAGAGGG116250.066.133872
TAAGGGA28900.066.020083
GACCGAT2000.065.792438
AGAGGGC56100.065.507613
AGTACGG4100.065.437541
Produced by FastQC (version 0.11.5)