FastQCFastQC Report
Mon 27 Feb 2023
SRR3126481.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126481.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3341259
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319200.9553285153889597No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG43490.1301605173379256No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG36440.10906068640593261No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34630.10364356669147767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT101700.085.6584851
ACGGGAT8950.074.034964
ATAGCGG6400.073.497131
AGTAGGG55900.072.716352
TAGGGCA17150.071.518294
GTAGGGA25300.071.512343
ATAGGGA31850.071.41293
ATAGAGG26600.070.7340851
AGGGATG53200.069.607385
AGGGCAT34250.069.5645755
AGGGTAC12800.068.655035
GTAGGGC18200.068.4250643
AGACGGG23100.068.149172
TAGAGGG68600.068.0225452
TAGACGG5400.067.944021
AGAGGGC51650.067.783923
ATAGGGC21600.067.227273
AAGAGGG118700.066.8266142
GAGGGAT41150.066.6935354
AATAGGG49000.066.6525042
Produced by FastQC (version 0.11.5)