FastQCFastQC Report
Mon 27 Feb 2023
SRR3126480.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126480.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3741154
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405391.0835961310333657No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG49870.13330111511047127No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39890.10662485425620008No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG39100.10451320635290608No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37890.10127891019722791No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT117650.085.472741
TAGACGG5750.076.890351
TAGGGCA21050.073.90214
AGTAGGG66650.073.2649462
ACGGGAT10800.070.4972464
TAGAGGG72900.070.078192
GTACGGG10400.070.045342
AGGGCAT38550.069.003795
GTAGGGA30400.068.642053
AGGGATG56900.068.6387945
ATAGGGC22650.068.059153
GTAGGGC17700.067.974753
TAGCGGG18750.067.928012
TAAGGGA33950.067.693963
ATAGGGA35000.067.5430763
AGACGGG24850.067.5184862
TAGGGTA13300.067.49364
AGGGAAT38500.067.262315
GGTAAGG24500.067.191421
GTAGGGT15150.067.007283
Produced by FastQC (version 0.11.5)