FastQCFastQC Report
Mon 27 Feb 2023
SRR3126479.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126479.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024196
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157560.7783831210021164No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45200.22329853433165564No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30700.15166515495535018No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG30100.14870101511908926No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC27460.13565879983954124No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTTCCGAA25620.12656877100834107No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG25150.1242468614699367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54900.084.0938641
ACGGGTC2500.082.714024
CGTAGGG5500.080.379052
GTACGGG4700.080.051572
ATAGCGG3550.076.889551
AGGGATG27600.074.5815665
TAGCGGG10150.074.136422
AGGGAAT24250.071.8999565
CTACGAC9350.071.877165
TACGACC9350.071.877166
TCCGCTA9450.071.2150651
GTAGGGC9450.071.1165543
GTAGGGA18100.070.624733
GGTACGC409.758878E-870.49497
AGTAGGG33100.070.4743962
AGGGCAT17300.070.359085
TAGGGTA8850.070.096634
ATGCGGG10300.069.8605352
AAGGGCG6000.069.711634
TATAGGG15700.069.4969942
Produced by FastQC (version 0.11.5)