FastQCFastQC Report
Mon 27 Feb 2023
SRR3126478.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126478.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2094315
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160180.7648324153720907No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43780.20904209729672948No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG32110.15331982056185436No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31090.148449493032328No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC30140.14391340366659264No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG26990.12887268629599655No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTTCCGAA23450.11196978486999329No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57350.084.887631
GTACGGG5250.078.7691652
CGTAGGG6000.074.405532
AGGGATG29850.072.734575
AGTAGGG33800.071.7407462
TAGCGGG10900.071.5672762
AGGGCAT18950.070.925125
ACGGGAT6400.070.4911354
AGTACGG2900.069.744171
AGGGAAT23300.069.3818365
TAGGGCA11100.068.5859764
ATAGCGG3850.068.4171141
AGAGGGC27850.068.170963
CGAAGGG18300.067.793142
TAAGGGA18750.067.420853
ATAGAGG13900.067.34041
ATAGGGC12800.067.186863
AGGGTAT13200.066.930985
TAGGGTA8500.066.897484
GGATAGG13300.066.841721
Produced by FastQC (version 0.11.5)