FastQCFastQC Report
Mon 27 Feb 2023
SRR3126477.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126477.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2331874
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201830.8655270396256403No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC51520.2209381810509487No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35680.1530099825290732No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG34190.1466202719357907No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC32760.14048786512478806No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG30100.12908073077704885No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69500.084.911381
CGTAGGG7100.077.449782
ATAGCGG4550.075.442911
ACGGGTA3500.075.199114
TAGCGGG11500.073.564352
TAGGGCA13350.072.8755344
AGGGATG34050.072.46615
AGTAGGG40200.070.616072
GTAGGGC10400.070.047253
CTACGAC11250.069.768075
ACGGGAT6950.069.653854
GTAGGGT9650.069.6468353
AGGGCAT21850.069.477445
TCCGCTA11500.069.102831
TACGACC11350.068.739276
GAATAGG13600.068.459431
TAGGGTA11000.068.362834
TAGAGGG39750.068.104852
TATAGGG18000.067.8880842
AGAGGGC32750.067.4495853
Produced by FastQC (version 0.11.5)