FastQCFastQC Report
Mon 27 Feb 2023
SRR3126476.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126476.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080432
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294390.9556776452133986No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37210.12079474567203562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT103200.081.888341
CGTAGGG8650.072.844182
AGGGATG54950.072.1840445
ATAGGGC19450.069.588793
GGTAAGG19200.069.355471
TAGGGCA21400.069.177244
AGTAGGG58500.068.644892
TATAGGG25500.068.59762
TAAGGGA32150.068.558033
TAGCGGG17700.068.541342
ATAGCGG6550.066.8093261
ACGGGAT10700.066.761534
CGAAGGG29400.066.695252
AGAGGGC45750.066.5656743
ATAGGGA27850.066.149613
AGGGAAT36200.065.951035
TAGGGTC9650.065.7465444
TAGAGGG61000.065.67742
AGGGTAC15650.065.464915
ATAAGGG44550.065.335482
Produced by FastQC (version 0.11.5)