FastQCFastQC Report
Mon 27 Feb 2023
SRR3126475.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126475.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104591
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298390.9611249919876724No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38390.12365557975269528No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG31160.1003674880201611No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT107150.083.936331
CGTAGGG10400.071.3876652
ATAGCGG8400.069.99791
GGTAAGG20400.069.4049761
GTAGGGA27450.069.333013
AGGGATG54950.068.927855
AGTAGGG55050.068.371422
TAGAGGG60000.068.212872
AGTACGG4700.068.055831
ACGGGAT10850.067.565074
GTACGGG7350.067.127642
TAGGGCA19300.066.9580464
GAATAGG22650.066.871641
TAAGGGA33500.066.771253
ATAGGGC20900.066.10413
TAGCGGG20950.065.942092
AGAGGGC43150.065.560623
AGGGAAT35000.065.1180045
ATAGGGA29600.065.090793
TAGGGAT27800.064.910334
Produced by FastQC (version 0.11.5)