FastQCFastQC Report
Mon 27 Feb 2023
SRR3126474.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126474.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3505538
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380401.0851401411138604No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43450.12394673799000323No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG35190.1003840209405803No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT128500.084.3006741
CGTAGGG10250.076.115172
TAGGGCA20900.073.534044
ATAGCGG8550.073.168771
AGGGATG63450.070.8871155
ATAGGGC22550.070.029283
ATAGGGA35450.069.470763
GTAGGGA27800.068.300453
TAGAGGG65950.068.2712252
ACGGGAT11650.068.178554
TAAGGGA34950.068.044083
TAGCGGG21500.067.109952
GTAGGGT14450.066.351283
ATAACGG4750.066.3470151
GAATAGG22850.066.284181
AGTAGGG62200.065.9645842
AGGGAAT40150.065.90375
GGTAAGG22650.065.831121
AGAGGGC50700.065.8168953
AAGAGGG123500.065.608072
Produced by FastQC (version 0.11.5)