FastQCFastQC Report
Mon 27 Feb 2023
SRR3126473.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126473.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126534
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80690.7162677735425651No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC61660.5473425569046296No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTCAATCC25180.22351744377000607No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG24310.21579464090742045No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC20940.1858798757960257TruSeq Adapter, Index 22 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG20290.1801099656113353No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT12060.10705402588825548No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC11750.10430222256940314No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGTC11750.10430222256940314No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT33550.085.955311
CGTAGGG3250.082.4603962
AGGGATG33400.082.313395
CGGGTCA750.081.4597855
CGGGATG7750.081.2576455
TAGGGCG1400.080.564624
CGGGAAT3150.080.564625
GGATGGC26100.080.307397
GATGGCA23700.079.119898
GCGGGAT8500.078.511014
CGAGGGA9400.078.493363
TCCGCTA10200.078.406761
AGGGATC14050.077.936475
AAGGGAT34250.077.6635064
TACGACC10350.077.6456156
CGCTACG10300.077.566263
GCGTAGG1950.077.200491
AGTAAGG7750.077.091541
AGGGAAT21350.076.822555
TAGCGGG5450.076.7798842