Basic Statistics
Measure | Value |
---|---|
Filename | SRR3126473.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1126534 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 8069 | 0.7162677735425651 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 6166 | 0.5473425569046296 | No Hit |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTCAATCC | 2518 | 0.22351744377000607 | No Hit |
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG | 2431 | 0.21579464090742045 | No Hit |
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC | 2094 | 0.1858798757960257 | TruSeq Adapter, Index 22 (95% over 21bp) |
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG | 2029 | 0.1801099656113353 | No Hit |
AAAGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT | 1206 | 0.10705402588825548 | No Hit |
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC | 1175 | 0.10430222256940314 | No Hit |
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGTC | 1175 | 0.10430222256940314 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 3355 | 0.0 | 85.95531 | 1 |
CGTAGGG | 325 | 0.0 | 82.460396 | 2 |
AGGGATG | 3340 | 0.0 | 82.31339 | 5 |
CGGGTCA | 75 | 0.0 | 81.459785 | 5 |
CGGGATG | 775 | 0.0 | 81.257645 | 5 |
TAGGGCG | 140 | 0.0 | 80.56462 | 4 |
CGGGAAT | 315 | 0.0 | 80.56462 | 5 |
GGATGGC | 2610 | 0.0 | 80.30739 | 7 |
GATGGCA | 2370 | 0.0 | 79.11989 | 8 |
GCGGGAT | 850 | 0.0 | 78.51101 | 4 |
CGAGGGA | 940 | 0.0 | 78.49336 | 3 |
TCCGCTA | 1020 | 0.0 | 78.40676 | 1 |
AGGGATC | 1405 | 0.0 | 77.93647 | 5 |
AAGGGAT | 3425 | 0.0 | 77.663506 | 4 |
TACGACC | 1035 | 0.0 | 77.645615 | 6 |
CGCTACG | 1030 | 0.0 | 77.56626 | 3 |
GCGTAGG | 195 | 0.0 | 77.20049 | 1 |
AGTAAGG | 775 | 0.0 | 77.09154 | 1 |
AGGGAAT | 2135 | 0.0 | 76.82255 | 5 |
TAGCGGG | 545 | 0.0 | 76.779884 | 2 |