FastQCFastQC Report
Mon 27 Feb 2023
SRR3126472.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126472.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145675
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82560.7206232133894865No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC64130.5597573482881271No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG26400.23043184149082419No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC22970.20049315905470577TruSeq Adapter, Index 22 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTCAATCC22910.19996945032404478No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG21880.1909791171143649No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT12560.10962969428502847No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGTC12450.10866956161215004No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC11750.10255962642110547No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT36550.084.936461
AGGGATG34700.083.1494455
GCGGGAT8400.080.557074
CGCTACG10200.080.165673
AAGGGAT33750.079.781334
GGATGGC28450.079.77847
TCCGCTA10450.078.769741
TAGGGCG2450.078.6390464
CGAAGGG12000.077.927772
AAGGGAC14450.077.3979644
CGGGATG7900.077.327985
GTACGGG2100.076.081672
TAGACGG1300.075.98251
AGGGATC14650.075.6998145
AGGGCAT13100.075.688795
TAAGGGA16550.075.530613
GATGGCA26400.075.471388
GGACCGT750.075.186597
GCTACGA10850.074.9267354
CGGGATA2700.074.838515
Produced by FastQC (version 0.11.5)