FastQCFastQC Report
Mon 27 Feb 2023
SRR3126470.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126470.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976305
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242921.2291625027513466No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26400.13358262009153446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT87200.086.450371
TAGGGCA9900.070.258294
AGTAGGG35750.069.977672
ATAGGGC9950.069.905243
GAATAGG11000.068.87381
AGGGATG29800.068.130035
ATAGGGA18200.067.396943
TGTAGGG16350.066.438242
GTAGGGC11050.066.348853
AGAGGGC29300.065.763863
ATGCGGG11600.065.6721042
TAGGGCG3650.065.667184
GAGGGAT24200.065.640584
GTACGGG5550.065.241152
AGGGCAT16200.064.693545
GTAGGGA15550.064.677683
TAGAGGG36000.064.397392
TAGGGTC6350.064.389734
TAAGGGA17000.064.137233
TATAGGG14900.064.066892
Produced by FastQC (version 0.11.5)