FastQCFastQC Report
Mon 27 Feb 2023
SRR3126469.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126469.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978663
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241231.2191565718871784No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25370.12821789258706512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86600.087.279611
ATAGGGC10250.075.648713
ACGGGAT4400.071.558894
AGGGATG30650.069.915885
AGTAGGG35100.069.7509462
TAGGGCA9750.069.406374
ATAGGGA18350.068.3780443
GTACGGG4950.068.35132
ATAGCGG5000.066.788711
GTAGGGA14800.066.680533
GTAGGGC10300.066.61273
GAATAGG12400.065.9997561
CGTAGGG5500.065.788132
ATAAGGG27950.065.7376862
ATGAGGG32950.065.602742
TAAGGGA16700.065.2849353
AATAGGG30850.065.194082
TAAGAGG17350.065.061871
TAGGGAT13400.064.879634
GAGGGAT27600.064.872014
Produced by FastQC (version 0.11.5)