FastQCFastQC Report
Mon 27 Feb 2023
SRR3126468.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126468.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220717
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302991.3643791622255335No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29360.13220955214014213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT104300.086.309331
ATAGGGC13200.075.8390353
GTAGGGC11700.072.70763
TAGGGCG4300.072.137764
GAATAGG14500.069.4138261
TAGGGCA11350.069.152484
AGTAGGG40900.068.0276642
ATAGGGA21850.067.540673
AGAGGGC30750.067.250373
TAGAGGG41750.067.092962
AATAGGG35400.066.3825452
TAAGGGA19650.066.013643
TACGGGT1500.065.798383
TAGGGAT15150.065.457134
AGGGATG33350.065.3896945
AAGAGGG79450.064.597532
GTAGGGA17400.064.555883
GATAGGG33200.064.26952
TAAGAGG18450.064.239821
ATGCGGG12550.064.038262
Produced by FastQC (version 0.11.5)