FastQCFastQC Report
Mon 27 Feb 2023
SRR3126467.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126467.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118153
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99360.8886082673838016No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC50060.447702595262008No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG19290.17251664128254363No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC19260.17224834168490358No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG18060.16151635777930212No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT17830.15945939419739516No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCACGGTGT16200.14488178272561986No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT33850.084.903861
ACGGGTA1550.084.89894
CTAACGG800.082.315751
TAGACGG1850.081.362281
AGGGATG24400.080.897525
ATAGCGG3000.079.963871
ACGGGAT3500.077.8817444
CTACGAC9100.076.435675
TCCGCTA9550.075.8511661
TAGGGTA3850.075.684464
GGATGGC22600.074.863457
TAAGGGA9650.073.0532763
GTAGGGT3800.072.969973
AGTAGGG17300.072.818572
GGTAAGG5500.072.694431
TAGCGGG6550.072.482562
AGGGCAT8650.072.262225
AGGGTAT6200.072.012465
AGGGAAT16450.071.996345
CGAAGGG10750.071.71152