FastQCFastQC Report
Mon 27 Feb 2023
SRR3126465.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126465.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282833
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127980.997635701607302No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC61830.48198011744319014No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG23200.18084972868643073No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG21640.1686891434816535No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC21550.16798757125830097No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18490.14413411566431483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT44550.086.2586061
AGGGATG27850.078.134925
ATAGCGG2500.077.138631
ACGGGTA2100.076.093764
GAGGGAT21500.075.417144
TAAGGGA13450.075.128653
TAGCGGG6950.075.063292
AGGGCAT11900.075.040555
TAGGGCA8050.074.731464
TCCGCTA12050.074.5545961
GGATGGC25150.074.002537
TAGACGG1850.073.731721
AGTAGGG21050.073.6802752
CTACGAC11950.073.5466845
ACGGGAT4800.073.4360664
CGAAGGG11400.072.972262
GGAGCTA27800.072.862659
GTACGGG3900.072.306282
GGTAAGG7050.072.054761
TATAGGG9750.071.824242
Produced by FastQC (version 0.11.5)