FastQCFastQC Report
Mon 27 Feb 2023
SRR3126464.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126464.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099653
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283321.3493658237813582No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC32780.15612103523772738No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG25940.12354422373601734TruSeq Adapter, Index 22 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG24010.11435222867778629TruSeq Adapter, Index 22 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT23570.1122566443121792No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23290.1109230906249747No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98750.087.0093151
TAGACGG2500.077.169541
CGTAGGG5800.076.20642
AGGGATG32800.072.21565
GAATAGG13650.072.046971
AGTAGGG32850.070.567242
TAAGGGA21300.069.944573
AAGGGAT40100.069.734374
TAGGGCA11400.069.671664
GAGGGAT29100.069.60794
ATAGGGA22150.069.594423
ACGGGAT7950.069.166064
TAGCGGG12400.069.014652
TAGGGTC5600.068.8176964
ATAGGGC12950.068.590873
AGGGTAC8950.067.739355
TAGGGAT16150.067.5133744
AGGGAAT25150.067.4595645
AGAGGGC29700.066.619283
AATAGGG32750.066.619022
Produced by FastQC (version 0.11.5)