FastQCFastQC Report
Mon 27 Feb 2023
SRR3126463.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126463.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141709
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286291.3367362232684272No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC35460.16556871171573728No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG29240.13652648422358032TruSeq Adapter, Index 22 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG25930.121071536796082TruSeq Adapter, Index 22 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23390.10921184904205006No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT23370.1091184656739081No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99700.086.854091
AGGGATG33050.072.800535
ATAGCGG4850.072.7363051
CGTAGGG5950.071.8704452
TAAGGGA21950.071.078723
GAATAGG13900.071.06181
TAGGGAT15450.070.261854
GTAGGGC10050.070.139273
GAGGGAT31400.070.0409854
TAGGGCA9700.069.27884
AGGGACT30250.068.975315
AGTAGGG34900.068.805222
ATAGGGA20600.068.436863
ATAGGGC12050.068.247553
AAGGGAT39450.068.01824
AGGGAAT23500.067.5903855
ATAGAGG14700.067.514461
ATGAGGG32800.067.0498662
AGGGATC20400.066.804225
CGAAGGG23150.066.7837452
Produced by FastQC (version 0.11.5)