FastQCFastQC Report
Mon 27 Feb 2023
SRR3126462.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126462.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2392471
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358451.4982417759713702No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC39450.16489228082597449No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG30280.1265637075642714TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG29900.12497539155124555TruSeq Adapter, Index 22 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26330.11005358058676573No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT24730.10336593421613052No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT119550.087.859871
ATAGGGC12600.074.603073
TAGGGCA12550.074.15134
TAGACGG2450.072.957331
ACGGGAT7000.072.514184
AGGGATG38100.072.411985
AGGGAAT28400.072.154845
TAGGGCG3600.071.8054664
ATAGAGG17900.071.739941
GTAGGGC10500.071.171333
TAGGGAT16650.071.135044
GAATAGG14550.071.123171
ATAGGGA22800.070.9121863
AGTAGGG36900.070.436222
GAGGGAT36600.070.243084
TAGAGGG43050.070.09052
ACGGGTA3900.069.897354
TAAGGGA23200.068.6766363
TAACGGG8450.068.414112
AGAGGGA61400.067.438033
Produced by FastQC (version 0.11.5)