FastQCFastQC Report
Mon 27 Feb 2023
SRR3126461.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126461.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625407
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227170.8652753649243717No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT49520.18861837421778796No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76250.082.7480851
TACGGGT2100.076.09083
ATAGGGA23550.070.246443
TAGGGCA12800.070.128724
AGTAGGG45350.069.768472
AGGGATG40050.069.586455
ATAGAGG18200.069.542451
CGTAGGG7600.069.282872
GAATAGG15350.068.967291
TAGGGAT16900.068.132124
AGGGCAT22350.067.078865
TAAGGGA25450.067.033423
ATAGGGC16450.066.853243
ACGGGAT5800.066.44444
ATAAGGG39200.066.322442
GTACGGG6600.066.246122
AGAGGGC36550.066.220493
AGATAGG16850.065.899351
ATGAGGG41350.065.8300862
GATAGGG39550.065.4977652
Produced by FastQC (version 0.11.5)