FastQCFastQC Report
Mon 27 Feb 2023
SRR3126460.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126460.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2661154
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226870.8525248820624436No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT47970.18026014278016228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75650.084.299551
ATAGGGA21500.072.5615843
TAGGGCA13650.071.2598344
ACGGGAT6500.070.123814
AGTAGGG45100.070.016422
TAATAGG7600.069.92611
AGGGATG40400.069.903725
GGTAAGG17200.069.177771
TAAGGGA25700.068.931123
GAATAGG15150.068.6047441
ATAGAGG16950.068.533311
AGGGAAT28300.068.409745
CGTAGGG6550.068.15372
ATAAGGG40100.067.379942
ATAGGGC15100.067.217743
TAGGGAT17400.066.974514
AAGGGGC81800.066.406664
AATAGGG37650.066.397812
AGAGGGC35250.066.252823
AAGAGGG88550.066.226712
Produced by FastQC (version 0.11.5)