FastQCFastQC Report
Mon 27 Feb 2023
SRR3126459.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126459.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2970613
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286880.9657266025564419No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT54900.18481034049201295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93700.084.737171
CGTAGGG8000.074.022432
TAGGGTA8750.072.5117654
AGGGATG45700.070.9603355
ATAGAGG20250.070.381531
TAGGGCA16450.069.426164
ATAGGGA26900.069.36193
TAAGGGA28100.069.07593
AGTAGGG52900.068.942782
ACGGGAT7100.068.1807254
ATAGGGC19100.067.913873
ATGTAGG10200.067.788861
TGTAGGG24200.067.778632
AAGAGGG98100.067.3595352
AAGGGAT49850.067.315614
TGAAGGG57100.066.670192
CGAAGGG34750.066.406332
TAGAGGG53650.065.876552
ATAAGGG44100.065.755092
GAATAGG18300.065.543591
Produced by FastQC (version 0.11.5)