FastQCFastQC Report
Mon 27 Feb 2023
SRR3126458.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126458.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113015
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222961.055174714803255No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28670.13568289860696683No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82050.086.158161
ACGGGAT6800.081.5531164
CGTAGGG6650.071.407192
ATAGGGC12050.070.982583
ATAGCGG4600.070.598641
TAGGGCA10550.070.38374
ATAACGG2550.070.1372151
AGGGATG33900.069.4553155
AGTAGGG39050.069.108752
CGAAGGG22600.069.067212
AGGGACT27100.068.500745
GTACGGG4700.067.022282
GAGGGAT33100.067.016464
TAAGGGA20100.066.637133
TAAGAGG17100.066.0572051
TAGGGCG3350.065.9356844
ATAGAGG15050.065.673161
GTAGGGA16950.065.434953
AGAGGGC28150.064.443093
AGGGCAT17600.064.3534555
Produced by FastQC (version 0.11.5)