FastQCFastQC Report
Mon 27 Feb 2023
SRR3126457.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126457.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156510
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227641.055594455856917No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28880.13392008383916607No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79150.086.6387561
ATAGCGG4250.080.786641
ATAGGGC12350.071.913023
CGTAGGG7000.071.157472
GAGGGAT31650.069.333924
ACGGGAT6150.068.76544
TAGAGGG43950.068.748752
AGGGATG36000.068.1350565
TAGGGCA10800.067.438914
AGTAGGG37300.066.895722
TATAGGG17450.066.783462
GTACGGG5300.066.496392
AGGGATC18350.066.067255
GTAGGGA17200.065.8417363
ATGAGGG36550.065.825672
TAAGGGA20050.065.388673
AGGGACT28150.065.2681055
ATAACGG1950.065.123051
AAGGGAT42350.064.7980654
ATAGAGG17500.064.771591
Produced by FastQC (version 0.11.5)