FastQCFastQC Report
Mon 27 Feb 2023
SRR3126455.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126455.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818814
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146400.8049201292710525No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33070.18182178056689688No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31280.17198020248359647No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT47350.084.568541
CGTAGGG5000.078.06072
TAGCGGG12300.076.080322
AGGGATG40850.075.930025
ACGGGAT6750.073.8012544
ATAGCGG4500.073.1955641
GTAGGGA16400.073.073173
GAGGGAT28850.072.1638264
GGATGGC35600.071.946257
TAGGGCA11700.071.096644
GAATAGG11300.070.789641
AGACGGG12050.070.634352
TAAGGGA17100.069.8070453
AGTAAGG12050.069.1170961
AGGGTAC8300.069.0785755
ATAGGGA17750.068.574563
CGGGATG9250.068.080815
AAGGGTA12000.067.7526864
ATAGGGC12500.067.674363
AAGAGGG63200.067.486142
Produced by FastQC (version 0.11.5)