FastQCFastQC Report
Mon 27 Feb 2023
SRR3126454.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126454.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886527
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148400.7866306710691128No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35650.18897158641249237No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32770.17370543861815918No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG19100.10124424405269578No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT48200.083.81761
CGTAGGG5950.081.349022
AGGGATG44200.075.063025
ACGGGAT5950.073.453014
GGTAAGG11450.072.292991
AGGGAAT23650.072.130535
GGATGGC39650.071.5894247
GAGGGAT27400.070.319694
GTAGGGA15750.070.1182253
AAGGGTA13950.070.07014
GAATAGG11500.069.933841
ATGGCAC37400.069.739139
ATAGGGA17750.068.836473
AAGGGAT35600.068.7751164
TAGGGCA11350.068.317454
CGGGATG8600.068.305425
AGTAGGG31900.068.058662
TAGGGCG3600.067.880414
TAAAGGG31850.067.722872
AGATAGG12250.067.571821
Produced by FastQC (version 0.11.5)