FastQCFastQC Report
Mon 27 Feb 2023
SRR3126453.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126453.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139070
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187490.8765024052508801No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC42340.1979364864169943No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37060.1732528622251726No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61000.085.286851
AGGGATG44250.078.2808465
CGTAGGG6250.074.448592
TAGGGCA14000.073.8577354
TAGCGGG12550.073.402982
GGATGGC42950.072.439567
GTAGGGA18150.071.73063
ATAGGGA19850.071.033313
ATAGGGC15200.070.809773
GGTAAGG15100.070.714051
GAGGGAT33300.070.4299854
ACGGGAT7300.070.178644
TAGGGCG2950.070.102254
ATAGCGG4500.070.035641
AGTAGGG37150.069.583332
GAATAGG13500.069.338771
AAGGGAT39200.068.941884
AAGAGGG73100.068.2180562
AGGGAAT27300.068.004215
TAGGGAT17500.067.680544
Produced by FastQC (version 0.11.5)