FastQCFastQC Report
Mon 27 Feb 2023
SRR3126452.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126452.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1971280
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170150.8631447587354408No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC54290.2754048131163508No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG30130.1528448520758086No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28820.1461994237246865No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC27310.13853942615965262No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG24570.12463982792906131No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGGACTGG22970.11652327421776713No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54850.084.7608261
CGTAGGG6200.081.1415252
TAGGGCA13500.076.5885244
GTAGGGC9850.074.909723
AGGGATG35800.073.646925
TAGCGGG9650.072.108332
GGTAAGG11200.071.424271
AGTAGGG30900.071.209582
TAAGGGA20100.070.846983
ACGGGAT6900.070.8368154
AGGGTAC9300.070.7489245
AGGGCAT20300.070.148985
AAGGGAT39300.070.077694
ACGGGTA2150.069.949774
TAGGGTA6200.069.7382354
GGATGGC29250.069.411697
ATAGCGG4000.069.407581
CTACGAC11750.069.196335
AGGGAAT24950.069.13065
GTAGGGA15300.068.80683
Produced by FastQC (version 0.11.5)