FastQCFastQC Report
Mon 27 Feb 2023
SRR3126450.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126450.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2279635
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217880.955767041653598No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC65850.288862032737697No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG34540.1515154838384215No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32840.14405814965992364No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC29530.12953828134767187No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG28320.12423041407944692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68150.086.1249851
CGTAGGG6950.079.1182252
AGGGATG41950.076.742175
TAGGGCG4050.076.5886464
TAGGGCA13850.075.671194
ACGGGAT6600.075.480964
TAGGGAT20100.072.951464
GTAGGGC10650.072.371853
GGTAAGG12350.072.354751
TAGGGTA8550.072.007984
AGGGTAC10400.071.8520665
AGTAGGG35500.071.754052
GAGGGAT32150.071.3369144
GTAGGGA21150.071.1074453
ATAGGGA24150.070.836923
TAGCGGG12000.070.104712
CTACGAC13550.070.0628055
AGGGCAT21250.069.666995
CGAGGGA11300.069.040693
AAGGGAT45450.068.971144
Produced by FastQC (version 0.11.5)