FastQCFastQC Report
Mon 27 Feb 2023
SRR3126449.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126449.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526425
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387161.5324420871389415No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34510.1365961783943715No Hit
CGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT30550.12092185598226743TruSeq Adapter, Index 10 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC27830.11015565472950908TruSeq Adapter, Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG26700.10568293141494405RNA PCR Primer, Index 10 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT133000.086.159571
TAGGGCA12000.073.23434
TAGGGTA8500.071.8751144
ATAGGGC15600.070.794163
AGGGATG33450.070.668525
AGTAGGG41450.070.446912
TAGCGGG13500.070.3578342
CGTAGGG5700.070.119522
ACGGGAT6450.069.946454
ATAGGGA21800.069.199463
CGAAGGG23900.068.662942
ACGGGTA2850.067.607234
AGAGGGC38650.067.118733
ATAGAGG17900.067.041441
GATAGGG35800.066.1976242
CGAGGGA15600.065.672883
ATGAGGG39600.065.426132
GTAGGGA17150.064.94393
AAGAGGG78000.064.805032
GTAGGGT9000.064.749043
Produced by FastQC (version 0.11.5)