FastQCFastQC Report
Mon 27 Feb 2023
SRR3126448.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126448.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589756
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387531.4963957994498325No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33710.13016670296352242No Hit
CGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT30490.11773309917999997TruSeq Adapter, Index 10 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC29580.11421925463248275TruSeq Adapter, Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG28410.1097014544999606RNA PCR Primer, Index 10 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT26830.1036004936372384No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG26520.10240346967050178Illumina PCR Primer Index 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT131250.086.331541
CGTAGGG6350.071.785342
ACGGGAT5550.071.12944
ATAGGGC14950.070.7300953
GTAGGGA15550.070.418773
AGTAGGG39850.069.8121642
TAGGGCA11850.069.8002854
ATAGGGA19700.067.989453
AGGGATG32350.067.697795
TAGGGAT13800.066.748254
ATGCGGG11900.066.343762
AGAGGGC41850.066.1428153
TAGAGGG47250.066.138992
GTAGGGC11800.066.113323
TACGGGT1850.066.048743
TGTAGGG18350.065.81652
ATAGAGG17900.065.430211
AGGGAAT28350.065.3139655
CGAAGGG24650.065.19992
GTACGGG6650.065.013562