Basic Statistics
Measure | Value |
---|---|
Filename | SRR3126448.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2589756 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 38753 | 1.4963957994498325 | No Hit |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 3371 | 0.13016670296352242 | No Hit |
CGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT | 3049 | 0.11773309917999997 | TruSeq Adapter, Index 10 (95% over 21bp) |
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC | 2958 | 0.11421925463248275 | TruSeq Adapter, Index 10 (95% over 23bp) |
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG | 2841 | 0.1097014544999606 | RNA PCR Primer, Index 10 (95% over 22bp) |
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT | 2683 | 0.1036004936372384 | No Hit |
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG | 2652 | 0.10240346967050178 | Illumina PCR Primer Index 2 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 13125 | 0.0 | 86.33154 | 1 |
CGTAGGG | 635 | 0.0 | 71.78534 | 2 |
ACGGGAT | 555 | 0.0 | 71.1294 | 4 |
ATAGGGC | 1495 | 0.0 | 70.730095 | 3 |
GTAGGGA | 1555 | 0.0 | 70.41877 | 3 |
AGTAGGG | 3985 | 0.0 | 69.812164 | 2 |
TAGGGCA | 1185 | 0.0 | 69.800285 | 4 |
ATAGGGA | 1970 | 0.0 | 67.98945 | 3 |
AGGGATG | 3235 | 0.0 | 67.69779 | 5 |
TAGGGAT | 1380 | 0.0 | 66.74825 | 4 |
ATGCGGG | 1190 | 0.0 | 66.34376 | 2 |
AGAGGGC | 4185 | 0.0 | 66.142815 | 3 |
TAGAGGG | 4725 | 0.0 | 66.13899 | 2 |
GTAGGGC | 1180 | 0.0 | 66.11332 | 3 |
TACGGGT | 185 | 0.0 | 66.04874 | 3 |
TGTAGGG | 1835 | 0.0 | 65.8165 | 2 |
ATAGAGG | 1790 | 0.0 | 65.43021 | 1 |
AGGGAAT | 2835 | 0.0 | 65.313965 | 5 |
CGAAGGG | 2465 | 0.0 | 65.1999 | 2 |
GTACGGG | 665 | 0.0 | 65.01356 | 2 |